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Ultramer™ RNA Oligonucleotides

Single-stranded RNA oligos up to 120 bases, desalted and delivered dry in tubes

Ultramer RNA Oligonucleotides are manufactured using proprietary synthesis methods that deliver high-quality, long RNA oligos.

Ordering

  • Each oligo is quality controlled by electrospray ionization mass spectrometry (ESI-MS)*
  • Increase hybridization precision in your experiments using longer RNA oligos (60−120 bases)
  • Supports RNA-related applications such as CRISPR and the generation of RNA controls and templates
  • Receive guaranteed quantities of 4, 20, or 80 nmol

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI-MS.

Ultramer RNA Oligos

Formulated to guaranteed quantities of 4, 20, or 80 nmol. Shipped dry, in tubes.

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Product details

IDT has developed proprietary synthesis methods to deliver high-quality, long RNA oligos up to 120 bases. Ultramer RNA Oligos can be used in a variety of RNA-related applications. These include, but are not limited to:

  • Single-guide RNAs (sgRNAs) for CRISPR
  • Long RNAs for RNA structural studies
  • RNA controls for PCR and qPCR

Ultramer RNA Oligos are desalted to remove small molecular impurities and formulated to 4, 20, or 80 nmol.

In addition, your oligos will be verified via ESI-MS* and quantitated by UV spectrophotometry.

*With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI-MS.

Using the RNA Oligo Entry tool

  • To enter individual RNA bases, insert "r" before the base (rA, rU, rC, rG)
  • To enter individual 2'-O-Methyl RNA bases, insert "m" before the base (mA, mU, rC, rG)
  • To quickly convert an entire oligo to RNA, enter your sequence as DNA using A, T, C, G and then click "Convert to RNA''. All bases in your sequence will be converted to RNA (rA, rU, rC, rG)

Product data

IDT employs proprietary equipment that permits rapid, high-quality synthesis of nucleic acids. However, this platform can be modified to a lower throughput mode that uses an "extra rich" synthesis cycle. Truncated byproducts of synthesis obscures mass spectra, making it difficult to deconvolute and identify the desired species. However, our mass spectrometry methods enable us to confidently QC your Ultramer RNA Oligos up to 120 bases (Figure 1,2). We provide ESI-MS quality documentation for every oligo we synthesize*. Overall, these improvements to our already established manufacturing methods allow you to acquire longer, purer oligos for your research.

* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be evaluated by ESI-MS.

Figure 1. QC data illustrate the quality of a sample 90mer Ultramer RNA Oligo. The measured molecular weight of the oligo matches its expected molecular weight, which is determined by the specific oligo sequence. Data generated from ESI­-MS instruments at IDT.

Figure 2. QC data illustrate the quality of a sample 120mer Ultramer RNA Oligo. The measured molecular weight of the oligo is within 0.01% of its expected molecular weight, which is determined by the specific oligo sequence. Data generated from ESI-MS instruments at IDT.

Services

To provide you with an exceptional experience when using our custom oligos, we offer a range of custom purification, formulation, and quality control services. To learn more about available services for Ultramer RNA Oligos, click here.

Large-scale synthesis

Custom RNA oligos can be ordered in bulk with the same high purity you expect from IDT. For further information about our large-scale synthesis capabilities, click here.

GMP and OEM

If you require oligos that are ISO 13485:2016 certified, or if you are interested in our third-party manufacturing services, please click here.

Frequently asked questions

GMP refers to products manufactured under ISO 13485: 2016 QMS. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations for their legal marketing.

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