Our team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
xGen Hybridization Wash v3 Kit will produce an improved AT/GC balance to better reflect the probe panel design and library input content. The buffer compositions have changed to minimize bias from hybridization, capture, and wash. Therefore, most users who switch from v1 & v2 to v3 may observe an increase in AT content relative to the GC content.
The post-capture AT vs. GC content is largely dictated by the input library and the panel. Therefore, the resulting GC content would be a combination of the interactions between the library, the panel, the workflow, and the post-capture amplification mix.